Research of the geophysical electromagnetic phenomena with seismic activity is important for hazard-resistant strategy. Many papers which indicate the existence of geophysical electromagnetic phenomena associated with earthquakes are reported frequently. Anomalous propagation in the radio waves is sometimes related with earthquakes. In previous paper, authors proposed a new concept to estimate the relation between earthquakes and anomalous line-of-sight propagation in VHF band by statistical approach. Event probability of the anomalous line-of-sight propagation increased just a few days prior to earthquakes. In this paper, we investigated a new relationship between anomalous fluctuations in the radio waves, which propagated from line-of-sight region, and occurrences of earthquake by using statistical analysis. Monitoring the strength of radio waves and detecting the anomalous fluctuations by using a new original method, we can obtain the high probability which indicates strong relation between the anomalous propagation and occurrences of earthquake. After the stochastic consideration, we can find out the anomalous fluctuations, which appear one day prior to earthquakes near the wave propagation path.